Aerosol Inhalation of Heat-Killed Clostridium butyricum CGMCC0313-1 Alleviates Allergic Airway Inflammation in Mice

Figure 3

The effect of aerosol inhalation of HKCB on autophagy in the lung tissues of allergic mice. (a) Expression levels of LC3B, Beclin1, and p62 in lung tissues, as examined by immunohistochemistry staining (magnification 200x). (b–d) Expression scores of LC3B, Beclin1, and p62 in lung tissues. (e) Expression levels of LC3B, Beclin1, and p62 in lung tissues were examined using western blotting. (f–h) Relative expression levels of LC3BII/LC3BI, Beclin1, and p62 in lung tissues. , , and versus the NC group; ^#, ^##, and ^### versus the OVA group. a.i.: aerosol inhalation; CB: Clostridium butyricum; HKCB: heat-killed CB.

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