lncRNA SNHG15 as a ceRNA modulates Osteoclast Differentiation, Proliferation, and Metastasis by Sponging miR-381-3p/NEK2 Axis

Figure 2

Effects of SNHG15 on osteoclast differentiation, proliferation, and movement of osteoclast precursors. (a) The transfection of sh-SNHG15 in 293T cells was detected by RT-qPCR; (b) cell morphology was observed by optical microscope; (c) CCK-8 was used to detect cell proliferation rate; (d) TRAP staining observed osteoclast of THP-1 cells; (e) rhodamine-labeled phalloidin staining observed the positive rate of F-actin. (f) Transwell detected cell migration; (g) Western blot was used to detect proteins expression of osteoclast markers CTSK, MMP-9, NFAT2, and TRAP. Compared with sh-NC group: ^&, ^&&&; compared with RANKL+sh-NC group: , , and ; compared with RANKL+pcDNA-NC group: ^#, ^##.

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