lncRNA SNHG15 as a ceRNA modulates Osteoclast Differentiation, Proliferation, and Metastasis by Sponging miR-381-3p/NEK2 Axis

Figure 3

SNHG15 regulates the expression of NEK2 through sponge miR-381-3p. (a) The database predicted the targeted binding sites of SNHG15 and miR-381-3p; (b) the targeting relationship between SNHG15 and miR-381-3p was verified by dual-luciferase reporter gene assay, compared with NC mimic group: ; (c) RIP experiments verified the targeting effect of SNHG15 on miR-381-3p, compared with PMA mimic group: ; (d) the database predicted the targeted binding sites of miR-381-3p and NEK2. (e) The targeting relationship between miR-381-3p and NEK2 was verified by dual-luciferase reporter gene assay, compared with NC mimic group: ; (f) the mRNA expression of NEK2 was detected by RT-qPCR, compared with NC mimic+RANKL group: , compared with NC inhibitor+RANKL group: ^△△△; (g) Western blot was used to detect the proteins expression of NEK2. Compared with NC mimic+RANKL group: , compared with NC inhibitor+RANKL group: ^△△.

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