lncRNA SNHG15 as a ceRNA modulates Osteoclast Differentiation, Proliferation, and Metastasis by Sponging miR-381-3p/NEK2 Axis

Figure 4

Effects of SNHG15/miR-381-3p/NEK2 axis on osteoclast differentiation, proliferation, and movement of osteoclast precursors. (a) Western blot was used to detect the proteins expression of NEK2; (b) the expression of miR-381-3p was detected by RT-qPCR; (c) The mRNA expression of NEK2 was detected by RT-qPCR; (d) CCK-8 was used to detect cell proliferation rate; (e) rhodamine-labeled phalloidin staining observed the positive rate of F-actin; (f) Transwell detected cell migration; (g) Western blot was used to detect proteins expression of osteoclast markers CTSK, MMP-9, NFAT2, and TRAP; compared with sh-NC group: ; compared with NC mimic+RANKL group: ^△, ^△△, ^△△△; compared with miR-381-3pmimic + RANKL group: ^#, ^##; compared with sh-NC + RANKL group: ^&, ^&&; compared with sh-NEK2 + RANKL group: ^○, ^○○, and ^○○○.

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