Calreticulin as an Adjuvant In Vivo to Promote Dendritic Cell Maturation and Enhance Antigen-Specific T Lymphocyte Responses against Melanoma

Figure 3

CRT/39-272 exerts an adjuvant effect on OVAp-induced T cell differentiation and function. C57BL/6 mice were immunized subcutaneously with OVAp or OVAp-CRT on days 0, 7, and 14. Splenocytes harvested from immunized mice on day 21. (a) Splenocytes were restimulated with or without SIINFEKL (0.5 μg/mL) for 24 h. IFN-γ secreting cells were determined by IFN-γ ELISpot assay. (b) Splenocytes were restimulated with OVA or BSA (10 μg/mL) for 3 days. Cell proliferation from restimulated splenocytes was measured. (c) Splenocytes were restimulated with or without OVA (10 μg/mL) for 24 h, and then, CFSE-labeled B16 or B16-OVA cells were added. After 4 h coculture, the cells were removed, and the dead tumor cells were determined by flow cytometry. The results were replicated three times with a consistent trend. Data are representative of at least three independent experiments. . . .

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