The Pharmacologically Active Alkaloid Cryptolepine Activates a Type 1 Interferon Response That Is Independent of MAVS and STING Pathways

Figure 2

Cryptolepine increases JAK1, TYK2, STAT1, STAT2, IRF9, and OAS3 mRNA levels in THP1-derived macrophages. The derived macrophages from the parent THP1 cells were cultured with cryptolepine (CRYPT), fludarabine (FLUD), or IFN-α as indicated. The target genes were assessed after 24 h by RT-qPCR with gene-specific primers and probes using β-actin as an endogenous control. Data are shown as the means, from three varied experiments with each done in triplicate, and error bars represent the standard deviations. , , and (one-way ANOVA and Bonferroni’s test).

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