The Pharmacologically Active Alkaloid Cryptolepine Activates a Type 1 Interferon Response That Is Independent of MAVS and STING Pathways

Figure 4

Cryptopine activates IFN-1 response in MAVS and STING-deficient THP1-derived macrophages. The derived macrophages from the MAVS (a) and STING (b) deficient THP1 cells were cultured with cryptolepine (CRYPT), Poly(I:C), HSV-60, or IFN-α. The derived macrophages from the parent THP1 cells served as the control to which the IFIT1 induction in the deficient cells was compared. Also, the cytotoxic effects of cryptolepine on the MAVS (c) and STING (d) deficient cells were assessed. Data are shown as the means, from three varied experiments with each done in triplicate, and error bars represent the standard deviations. ; ns: difference not significant (Student’s -test).

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