The Pharmacologically Active Alkaloid Cryptolepine Activates a Type 1 Interferon Response That Is Independent of MAVS and STING Pathways

Figure 6

Cryptolepine activates IFN-1 response through IFNAR1 and STAT1 in THP1-derived macrophages. The derived macrophages from the parent THP1 cells were cocultured with the anti-IFNAR1 blocking antibody (aIFNAR1) and IFN-α (a) or cryptolepine (CRYPT) (b), and fludarabine (FLUD) and IFN-α (c) or cryptolepine (d) and the IFIT1 induction was evaluated after 24 h. Data are shown as the means, from three varied experiments with each done in triplicate, and error bars represent the standard deviations. , , , and (one-way ANOVA and Bonferroni’s test (a and c) or Student’s -test (b and d)).

(a)

(b)

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(d)