The Pharmacologically Active Alkaloid Cryptolepine Activates a Type 1 Interferon Response That Is Independent of MAVS and STING Pathways

Figure 7

Cryptolepine enhances IFN-1 response activation by Poly(I:C), HSV-60, and IFN-α in THP1-derived macrophages. The derived macrophages from the parent THP1 cells were cultured with cryptolepine and 50 IU/mL IFN-α (a), 0.5 μg/mL Poly(I:C) (b), and 0.5 μg/mL HSV-60 (c), and the IFIT1 induction was assessed after 24 h. Moreover, the macrophages were cocultured with fludarabine (FLUD), IFN-α (Poly(I:C) or HSV-60), and cryptolepine, and the IFIT1 induction was evaluated after 24 h (d). Data are shown as the means, from three varied experiments with each done in triplicate, and error bars represent the standard deviations. , , , and (Student’s -test).

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