Pentraxin 3 Facilitates Shrimp-Allergic Responses in IgE-Activated Mast Cells

Figure 2

PTX3 augments degranulation and production of proinflammatory mediators in IgE/Ag-activated RBL-2H3 cells. (a, b) RBL-2H3 cells were seeded on a 24-well plate and further incubated with DNP-IgE overnight. IgE-sensitized cells were preincubated with PTX3 (300 ng/ml) for 30 min and then stimulated with DNP-BSA for 15 min. Histamine content and β-hexosaminidase activity were determined as described in the Materials and Methods. (c) Effects of PTX3 on the mRNA levels of TNF-α, IL-4, IL-6, IL-13, and COX-2 in IgE/Ag-stimulated RBL-2H3 cells were determined by qPCR. mRNA level of β-actin was used as an internal control.

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