Control of Tumors by Antigen-Specific CD8⁺ T Cells through PDL1-Targeted Delivery of Antigenic Peptide

Figure 3

Activation of E7-specific CD8+ T cells by ID8-luc cells pretreated with PDL1-scFv-Fc or PDL1-scFc-Fc-RE7. (a) Representative flow cytometry analysis to demonstrate the activation of E7-specific CD8+ T cells following the incubation of ID8-luc cell pretreatment either 50 μg/mL of PDL1-scFv-Fc or PDL1-scFc-Fc-RE7. ID8-luc cells without treatment are included as a negative control. E7-specific CD8+ T cell activation was determined by CD8 and IFN-γ staining. (b) Representative bar graph depicting the % of IFN-γ-secreting E7-specific CD8+ T cells out of total E7-specific T cells (). values are indicated as one star () if , two stars () if , and three stars () if .

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