Control of Tumors by Antigen-Specific CD8⁺ T Cells through PDL1-Targeted Delivery of Antigenic Peptide

Figure 4

Characterization of the CTL-mediated killing of ID8-luc tumor cells incubated with the various chimeric proteins. (a) Representative luminescence imaging of in vitro E7-specific CTL killing of luciferase-expressing ID8-luc cells. ID8-luc cells were preincubated with 5 μg/mL PD-L1-scFv-Fc, PD-L1-scFv-Fc-RE7, or hIgG. Pretreated cells were then incubated with E7-specific CD8+ T cells (). CTL-mediated tumor cell death was determined by decreasing luminescence activity. (b) Bar graph depiction of tumor cell viability after treatment with PD-L1-scFv-Fc, PD-L1-scFv-Fc-RE7, or hIgG protein () (data representative of three experiments).

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