Research Article

Neoadjuvant Chemotherapy Improves the Immunosuppressive Microenvironment of Bladder Cancer and Increases the Sensitivity to Immune Checkpoint Blockade

Table 2

Molecular changes after neoadjuvant chemotherapy.

PathwayGene countGene

UpregulatedLymphocyte infiltrate10CCL21, CCL2, CXCR4, PTPRC, IL-10RA, FYB, CD52, SRGN, TYROBP, TNFAIP8
Tumor marker4EGR3, MMP2, PTGS2, ZEB1
Cytokine signaling4IL-6, IL-4, CSF1R, CSF2RB
Checkpoint pathway4CD69, PDCD1LG2, CD28, ENTPD1
TCR coexpression3ITK, CD3E, IL-7R
Adhesion_migration3NCAM1, ITGA1, ADGRE5
Innate_immune_response2LYZ, AXL
Antigen_presentation2CD1C, CD83
Type_II_interferon_signaling1CX3CL1
T_cell_regulation1KLF2
T_cell_differentiation1EGR2
PD_1_signaling1FOXO1
NK_cell_marker1NCR3
NK_activation1KLRG1
Myeloid_marker1MPO
Lymphocyte_development1IKZF1
Lymphocyte_activation1SH2D1A
Leukocyte_migration1VCAM1
Dendritic_cell1NRP1

DownregulatedProliferation5CCNB2, TOP2A, MAD2L1, KIAA0101, MELK
Neutrophil2KREMEN1, DGAT2
Checkpoint_pathway1TNF
Antigen_processing1HLA-C
Tumor_antigen1BAGE