Adjuvant Efficacy of the ECMS-Oil on Immune Responses against Bordetella bronchiseptica in Mice through the TLR2/MyD88/NF-κB Pathway

Figure 1

The levels of serum IgG and its subclasses. The horizontal and vertical coordinates of the graph stand for the level of antibody and different groups. Different letters above the bar indicate the presence of significant differences among the groups (). Groups A1, A2, and A3 stand for (ECMS200 μg-oil, ECMS 400 μg-oil, and ECMS800 μg-oil) group. Group B stands for oil group, group C stands for EMCS800 μg group, group D stands for phosphate buffer saline group. Serum IgG level during 2 weeks post the first immunization was significantly increased in ECMS-oil groups (A1, A2, A3) compared with oil group (B), ECMS group (C), or control group (D) (). Meanwhile, ECMS 400 μg-oil group (A2) had a significantly higher level of serum IgG1 than any other groups during 2 weeks after the first and second immunization (). Moreover, groups A1, A2, and A3 had a markedly higher level of serum IgG2b than any other groups (B, C, D) during 2 weeks post the second immunization (), while serum IgG3 level during 2 weeks post the first and second immunization was substantially elevated in groups A1 and A2 as compared to any other groups (A3, B, C, and D) ().