Adjuvant Efficacy of the ECMS-Oil on Immune Responses against Bordetella bronchiseptica in Mice through the TLR2/MyD88/NF-κB Pathway

Figure 4

The results of western blot TLR2, MyD88, NF-κB, and TNF-α, IL-12 concentration from Raw264.7 cell supernatant. Different letters above the bar indicate the presence of significant differences among the groups (). The horizontal and vertical coordinates of the graph stand for the level of cytokines and different groups in (a). The horizontal and vertical coordinates of the graph stand for the relative transcript levels of cytokines and different groups in (b) or level of cytokines and different groups in (b). Western blot analysis revealed that induction with 50 ng/mL or 25 μg/mL ECMS led to an increased expression of TLR2, while the expression of MyD88 and NF-κB was increased in the macrophages induced with 50 ng/mL ECMS (a). RT-PCR assay showed that while MyD88 expression was increased in ECMS-induced macrophages (50 ng/mL) as compared to cells in any other groups (), the expression level of NF-κB in ECMS-induced macrophages (50 ng/mL) was higher than that in lipopolysaccharide group () (b).

(a)

(b)