Adjuvant Efficacy of the ECMS-Oil on Immune Responses against Bordetella bronchiseptica in Mice through the TLR2/MyD88/NF-κB Pathway

Table 2

Real-time PCR primers.

No.	Gene	Sequence	Product length (bp)
1	GAPDH-F	CCCACAGTAAATTCAACGGCAC	87
	GAPDH-R	CATTGGGGTTAGGAACACGGA
2	IL4-F	CTAGTTGTCATCCTGCTCT	102
	IL4-R	AATAAAATATGCGAAGCACCT
3	IL10-F	CTCCCCTGTGAAAATAAGAGC	105
	IL10-R	GACTCAATACACACTGCAGGT
4	TLR2-F	CATGAAAAGCCTTGACCTGT	92
	TLR2-R	AGAATAAAAGGCGTCTCCCTC
5	TLR4-F	CCATCATTATGAGTGCCAA	85
	TLR4-R	AAGCCAAGAAATATACCATCG
6	IL-1 beta-F	ATTAGACAACTGCACTACAGG	103
	IL-1 beta-R	ATACTTTAGGAAGACACGGAT
7	ASC-1-F	GAAGCTGCTGACAGTGCAAC	112
	ASC-1-R	AGGAGGAACAGTTAAGCGCC
8	NF-kb-F	CTTCGATGGCACTGCTGTCA	81
	NF-kb-R	TGGAGTTCCTGGTAGAACGC
9	MyD88-F	AGAGCTGCTGGCCTTGTTAG	96
	MyD88-R	AAGCACGTTTCCTCACCGAT