Sesamin Protects against APAP-Induced Acute Liver Injury by Inhibiting Oxidative Stress and Inflammatory Response via Deactivation of HMGB1/TLR4/NFκB Signal in Mice

Figure 2

Sesamin attenuated APAP-induced hepatic cellular apoptosis in mice: (a) representative images of TUNEL staining, black arrows indicate the apoptotic hepatocytes (brown staining for the nuclei); (b) quantification of TUNEL-positive hepatocytes; (c) hepatic caspase three activity, one-fold is the amount of enzyme that will cleave 1.0 nmol of the colorimetric substrate Ac-IETD-pNA per hour at 37°C under saturated substrate concentrations; (d) hepatic caspase eight activity; (e) hepatic caspase nine activity. Data were expressed as mean ± SD; sesamin group mice were oral gavage with 100 mg/kg sesamin, APAP group mice were intraperitoneally injected with 350 mg/kg APAP for 24 hr, sesamin + APAP group mice received oral gavages of sesamin 100 mg/kg at 16.5, 8.5, and 0.5 hr for three times before 350 mg/kg APAP administration (biological repeats n = 6), ^## compared with the control group, , , compared with APAP group.

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