Sesamin Protects against APAP-Induced Acute Liver Injury by Inhibiting Oxidative Stress and Inflammatory Response via Deactivation of HMGB1/TLR4/NFκB Signal in Mice

Figure 4

Effect of sesamin on APAP-induced oxidative stress in mice: (a) hepatic DHE fluorescence detection (Ex/Em:535/610 nm, red fluorescent marker for the nuclei indicates ROS positive); (b) the expression of hepatic 3-NT was analyzed by IHC (black arrows indicates 3-NT positive); (c) hepatic MDA content; (d) hepatic SOD activity; (e) hepatic CAT activity. Data were expressed as mean ± SD; sesamin group mice were oral gavage with 100 mg/kg sesamin, APAP group mice were intraperitoneally injected with 350 mg/kg APAP for 24 hr, sesamin + APAP group mice received oral gavages of sesamin 100 mg/kg at 16.5, 8.5, and 0.5 hr for three times before 350 mg/kg APAP administration (biological repeats n = 6), ^#, ^## compared with the control group, , , compared with APAP group.

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