Sesamin Protects against APAP-Induced Acute Liver Injury by Inhibiting Oxidative Stress and Inflammatory Response via Deactivation of HMGB1/TLR4/NFκB Signal in Mice

Figure 7

Sesamin blunted APAP-induced HMGB1/TLR4/NF-κB activation in mice: (a) serum HMGB1 level was measured by ELISA; (b) the expression of hepatic TLR4/p-IRAK1/IRAK1/p-IκB/IκB/p-p65/p65 were detected by western blotting; (c) quantification of hepatic TLR4 protein expression; (d) quantification of hepatic p-IRAK1 protein expression; (e) quantitative analysis of hepatic p-IκBα expression; (f) quantitative analysis of hepatic p-p65 expression. Data were expressed as mean ± SD; sesamin group mice were oral gavage with 100 mg/kg sesamin, APAP group mice were intraperitoneally injected with 350 mg/kg APAP for 24 hr, sesamin + APAP group mice received oral gavages of sesamin 100 mg/kg at 16.5, 8.5, and 0.5 hr for three times before 350 mg/kg APAP administration (biological repeats n = 6), ^## compared with the control group, , , compared with APAP group.

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