MicroRNA-22 Regulates the Pro-inflammatory Responses and M1 Polarization of Macrophages by Targeting GLUT1 and 4-1BBL

Figure 2

miR-22 represses the expression of Slc2a1 and tnfsf9 and induced via TLR-mediated signaling pathways. (a and b) Control or miR-22 O/E RAW 264.7 cells were treated with LPS. (a) Slc2a1 or tnfsf9 mRNA levels were analyzed by qPCR. (b) Protein levels were determined by immunoblotting using anti-GLUT1 or 4-1BBL Abs. GAPDH levels were detected as an internal control. (c) BMDMs were treated with medium or TLR ligands for 4 hr, and miR-22 levels were measured by qPCR. (d) BMDMs were incubated with medium, PD98059 (PD, 10 μM), SB203580 (SB, 20 μM), SP600125 (SP, 20 μM), or Bay11–7082 (BAY, 10 μM) for 1 hr followed by LPS stimulation. After 4 hr, induction of miR-22 was analyzed by qPCR. Unstim, unstimulated. Data are shown as mean ± SD. N = 4, , , and ; ANOVA test or Student t test. Result shown is representative of three independent experiments.

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