MicroRNA-22 Regulates the Pro-inflammatory Responses and M1 Polarization of Macrophages by Targeting GLUT1 and 4-1BBL

Figure 3

miR-22 regulates the pro-inflammatory responses. (a) Control, miR-22 O/E, or miR-22-Inh RAW 264.7 cells were stimulated with LPS, and TNF levels were measured. (b) Control or miR-22 O/E RAW 264.7 cells were stimulated with medium or TLR ligands for 24 hr, and TNF and IL-6 concentrations were measured by ELISA. (c) Induction of Tnf or Il6 mRNAs in LPS-treated control or miR-22 O/E RAW 264.7 cells was determined by qPCR. Data are shown as mean ± SD. N = 4, , , , and ; Student t test (a–c). Result shown is representative of at least three independent experiments.

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