MicroRNA-22 Regulates the Pro-inflammatory Responses and M1 Polarization of Macrophages by Targeting GLUT1 and 4-1BBL

<div>Regulation of cell metabolism by miR-22. (a and b) Glycolysis (a) and mitochondrial respiration (b) were measured in LPS + IFN-<i>γ</i>- (a) or IL-4 (b)-stimulated control or miR-22-O/E RAW 264.7 cells on a seahorse XFe96 extracellular flux analyzer (<i>n</i> = 6). (c) Expression of metabolism genes was analyzed by qPCR. (d) Control or miR-22-O/E RAW 264.7 cells were treated with LPS for 24 hr and intracellular TG levels were determined. Total protein concentration was measured. <i>N</i> = 4. 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635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,53.846,0)"><path d="M113 -12C146 -12 170 11 170 46C170 78 146 103 114 103S58 78 58 46C58 11 82 -12 113 -12Z"></path></g><g transform="matrix(.013,0,0,-0.013,56.81,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,63.05,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,69.29,0)"><path d="M384 0V27C293 34 287 42 287 114V635C232 613 172 594 109 583V559L157 557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg>.</span> One-way ANOVA test. Result shown is representative of 2-3 independent experiments.</div>

Journal of Immunology Research

fig5

Figure 5

Figure 5: MicroRNA-22 Regulates the Pro-inflammatory Responses and M1 Polarization of Macrophages by Targeting GLUT1 and 4-1BBL 