Exosome Derived from Human Umbilical Cord Mesenchymal Cell Exerts Immunomodulatory Effects on B Cells from SLE Patients

Figure 4

miR-155 targets SHIP-1. (a) qPCR was used to detect the expression of miRNA-155 in B cells of the control group and SLE patients (n = 3 per group). U6 snRNA was used as an endogenous control. (b) The potential targets of miR-155 were predicted by integrating the results of three databases (TargetScan, miRDB, and miRWalk). (c) Conservation of the miR-155 target sequence in SHIP1 3′-UTR among different species and conservation of the miR-155 sequence among different species. (d) GEO chip GSE4588 analyzed the expression of SHIP-1. (e) The SHIP-1-related genes were screened for GO analysis and showed to be related to MAPK/ERK pathway. (f) The SHIP-1 expression was determined by qPCR (n = 3 per group). GAPDH snRNA was used as an endogenous control. (g) The dual-luciferase reporter assay was performed in 293 T cells. Cells were cotransfected with the wild- or mutant-type SHIP-1, 3′-UTR luciferase reporter plasmids, and miR-155 mimics or mimics-NC. The ratio of Renilla activity: Firefly activity represents luciferase activity. Data are expressed as the mean ± SEM. , .

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