SEMA5A-PLXNB3 Axis Promotes PDAC Liver Metastasis Outgrowth through Enhancing the Warburg Effect

Figure 2

Aberrant dual high SEMA5A and PLXNB3 expressions lead to poor prognosis. (a) Real-time PCR measured expressions of PLXN family in TAMs slightly or severely infiltrated metastatic niches ( samples per group). (b) IF staining on CK19 and PLXNA3 (top), PLXNA4 (middle), or PLXNB3 (bottom) in liver metastatic niches demonstrating PLXNs locations ( patients, 3 fields assessed per sample). CK19, green; PLXNs, red; DAPI, blue; scale bars, 50 μm. (c) IF staining on PLXNB3 and SEMA5A in liver metastatic niches displaying their colocation relationship ( patients, 3 fields assessed per sample). PLXNB3, green; SEMA5A, red; DAPI, blue; scale bars, 50 μm. (d) Relationships of PLXNB3 (left), PLXNA3 (middle), or PLXNA4 (right) mRNA expressions and SEMA5A were measured ( samples). (e, f) Heat map showing the coexpression situations of PLXNB3, PLXNA3 or PLXNA4, and CD163 (e) or SEMA5A (f) in liver metastatic niches (). (g) The Kaplan-Meier analysis on PDAC patients bearing dual high expressions of SEMA5A and PLXNB3 or single high expression of SEMA5A in liver metastatic niches ( patients per group).

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