SEMA5A-PLXNB3 Axis Promotes PDAC Liver Metastasis Outgrowth through Enhancing the Warburg Effect

Figure 3

SEMA5A-PLXNB3 axis promotes liver metastasis in vivo. (a) The coexpressions of CK19 and PLXNB3 were performed by IF staining in spontaneous PDAC liver metastasis KPC mouse model ( mice, 3 fields assessed per sample). CK19, green; PLXNB3, red; DAPI, blue; scale bars, 50 μm. (b) Real-time PCR measured mRNA levels of SEMA5A and PLXNB3 in human normal pancreas epithelial cell line HPNE, human PDAC cell line PANC1, CAPAN1, BxPC3, and AsPC1 and murine PDAC cell line KPC1199 and Panc02. (c, d) CT combined in vivo imaging with organ reconstruction assessed liver metastasis burdens of intrasplenic injection mouse model ( mice per group). Scale color bars: [4] [5]. Reconstructed liver, red; reconstructed spleen, orange; signals of tumor niches, rainbow. ; two tailed unpaired -test, . (e, f) Separated KPC1199^shCTRL or KPC1199^shPLXNB3 invaded mouse livers were assessed via in vivo imaging system ( mice per group). Scale color bars: [6] [7]. Rainbow, signals of tumor niches. ; two tailed unpaired t-test, . (g) IHC-P staining of PLXNB3 (left) or CD163 in liver metastasis of intrasplenic mouse models ( mice, 3 fields assessed per sample). Scale bars, 50 μm. (h) Ki67 and TUNEL staining on liver metastatic niches of intrasplenic injection mouse model were performed ( mice, 3 fields assessed per sample). TUNEL signals, green; Ki67, red; DAPI, blue; scale bars, 50 μm. (i) Prognosis of mouse model bearing KPC1199^shCTRL or KPC1199^shPLXNB3 injection was analyzed via the Kaplan-Meier curve ( mice per group).

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