SEMA5A-PLXNB3 Axis Promotes PDAC Liver Metastasis Outgrowth through Enhancing the Warburg Effect

Figure 5

SEMA5A-PLXNB3 axis facilitates the Warburg effects via enhancing glycolysis. (a, b) Relative mRNA level of HIF1A (a) or MYC (b) affected by administration of rSEMA5A or SEMA5A-ΔTSP ( repeats per sample; ; two tailed unpaired -test). ; ; NS: no significant difference. (c) IF staining in KPC derived ex vivo liver metastasis tissue treated with rSEMA5A or SEMA5A-ΔTSP demonstrating the expression and distributions of HIF-1α ( mice per group, 3 fields assessed per sample). HIF1A, green; DAPI, blue; scale bars, 50 μm. (d, e) Metabolism level alterations of PANC1 and BxPC3 cell lines affected by SEMA5A-PLXN3 axis were assessed by relative glucose consumption (d) and lactate production (e) ( repeats per group, ; two tailed unpaired -test). ; ; . (f, g) The expression alteration of key enzymes involved in glycolysis pathway in PANC1 (f) and BxPC3 (g) after stimulation by rSEMA5A or SEMA5A-ΔTSP exposure ( repeats per group, ; two tailed unpaired -test). , , and for rSEMA5A group; ^#, ^##, and for SEMA5A-ΔTSP group; NS: no significance. (h, i) Relative ECARs in PANC1 (h) or BxPC3 (i) treated with rSEMA5A or SEMA5A-ΔTSP measured by seahorse system ( repeats, ).

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