Development of a Novel Recombinant Full-Length IgY Monoclonal Antibody against Human Thymidine Kinase 1 for Automatic Chemiluminescence Analysis on a Sandwich Biotin-Streptavidin Platform for Early Tumour Discovery

Figure 4

Specificity and sensitivity of hTK1-IgY-rmAb#5: (a) SDS-PAGE detection of the purified antibody (0.5 μg). The IgY molecule heavy chain (H) was ≈66 kDa, and the light chain (L) was ≈25 kDa. (b) Western blot detection of antibody specificity; TK1-negative cell lines (143BTK1^-) and TK1-positive cell lines (HT29) were used, and GAPDH was used as a loading control. (c) ELISA titre detected the #5 antibody. Mr.: molecular weight marker.

(a) SDS-PAGE

(b) WB (native PAGE)

(c) ELISA titre