Development of a Novel Recombinant Full-Length IgY Monoclonal Antibody against Human Thymidine Kinase 1 for Automatic Chemiluminescence Analysis on a Sandwich Biotin-Streptavidin Platform for Early Tumour Discovery

Figure 5

TK1 IHC staining of hTK1-IgY-rmAb#5: (a) TK1 staining of normal tonsil tissue; (b) a patient with ovarian serous adenocarcinoma (grade 3). TK1 staining is observed in brownish-yellow, mainly in the cytoplasm (examples of TK1 in the red box). (c) Normal ovarian tissue was taken from the far outside of the total ovarian serous adenocarcinoma specimen as a standard control (normal spindle cells are shown in green box). Blue staining was used to counterstain nuclei with haematoxylin. Magnification 200x.

(a) Normal tonsillar tissue

(b) Tissue with ovarian carcinoma (grade 3)

(c) Normal ovarian tissue