Development of a Novel Recombinant Full-Length IgY Monoclonal Antibody against Human Thymidine Kinase 1 for Automatic Chemiluminescence Analysis on a Sandwich Biotin-Streptavidin Platform for Early Tumour Discovery

Table 1

The primers used in the construction of the phage display hen scFv library.

Primers	Sequences (5-3)	PCR steps
VH-B1	GTCATCAGGCCCAGCCGGCCATGGCCGCCGTGACGTTGGACGAGTCCGG	VH-1^st
VH-F1	GCCTGAGCCGCCACCACCGGAGGAGACGATGACTTCGGT
VL-B1-1	AGTGGTGGAGGAGGATCCCAGGCAGCGCTGACTCAGCC	VL-1^st
VL-B1-2	AGTGGTGGAGGAGGATCCCAGGCAGCACTGACTCAGCC
VL-B1-3	AGTGGTGGAGGAGGATCCCAGGCAGCGCTGACTCTGCC
VL-B1-4	AGTGGTGGAGGAGGATCCCAGGCAGCAGTGACTCAGCC
VL-B1-5	AGTGGTGGAGGAGGATCCCAGGCAGCACCGACTCAGCT
VL-F1	GTTACTGGTTTGCCTGCGGCCGCTAGGACGGTCAGGGTTGTCCC
VH-B2	CTTAGAGTCATCAGGCCCAGCCGGCCATGGCC	VH-2^nd
VH-F2	TCCTCCACCACTTCCGCCGCCGCCTGAGCCGCCACCACC
VL-B2	CGGCTCAGGCGGCGGCGGAAGTGGTGGAGGAGGATCC	VL-2^nd
VL-F2	CGATGGTTAGCGTTACTGGTTTGCCTGC
VH-B3	CTTAGAGTCATCAGGCCCAGCCGGCC	scFv-3^rd
VL-F3	CGATGGTTAGCGTTACTGGTTTG