Development of a Novel Recombinant Full-Length IgY Monoclonal Antibody against Human Thymidine Kinase 1 for Automatic Chemiluminescence Analysis on a Sandwich Biotin-Streptavidin Platform for Early Tumour Discovery

Table 4

Determination of the correlation score, stability, and accuracy of hTK1-IgY-rmAb#5 STK1p.

(a) STK1p assay. The correlation score was analysed in batches of hTK1-IgY-rmAb# 5 using the semiautomatic ECL dot blot assay with the BSA platform

Source of serum samples	value	Display range
	Pearson correlation	Different batch (OD)
Changzhou Cancer Hospital ()	0.811	0-1502
Shenzhen Charity Hospital ()	0.848	0-1438
Shenzhen Longhua Com. Hospital (W13, )	0.825	0-1722
Shenzhen Longhua Com. Hospital (W18, )	0.953	0-1638
Total ()	0.875

Shenzhen Longhua Community Hospital W13 and 18. : Pearson correlation score; display range indicates that the data are reliable in this range.

(b) HTK1 calibrators were used to evaluate the stability and accuracy in four batches of hTK1-IgY-rmAb#5 using the semiautomatic ECL dot blot assay with the BSA platform and the automatic chemiluminescence analyser with the sandwich-BSA platform. Pearson correlation score (, data not shown)

hTK1 calibrator (pM)	(OD value)	CV%
ECL dot blot
2.2		7.80
6.6		7.92
20		4.95
Automatic chemiluminescence
1		2.50
2		1.10
5		1.10
10		0.70
20		0.44