miR-21-5p Inhibits Ferroptosis in Hepatocellular Carcinoma Cells by Regulating the AKT/mTOR Signaling Pathway through MELK

Figure 1

High levels of miR-21-5p and MELK in hepatocellular carcinoma: (a) differential heatmap of miRNA expression in liver cancer; (b) volcano plot of miRNA expression in liver cancer; (c) RT-qPCR was used to detect miR-21-5p expression in pathological samples; (d) Western blot was used to detect MELK expression in pathological samples; (e) FISH for the level of miR-21-5p in liver cancer cells; (f) RT-qPCR for the level of miR-21-5p (normal liver cells QSG-7701, hepatoma cells HepG2, MHCC97H, MHCC97L, Huh-7, Hep3B); (g) Western blot for the level of MELK (normal liver cells QSG-7701, liver cancer cells HepG2, MHCC97H, MHCC97L, Huh-7, Hep3B). , , .

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