miR-21-5p Inhibits Ferroptosis in Hepatocellular Carcinoma Cells by Regulating the AKT/mTOR Signaling Pathway through MELK

<div>Overexpression of miR-21-5p and MELK can facilitate EMT in hepatocellular carcinoma cells: (a) transfection efficiency of miR-21-5p mimic by RT-qPCR; (b) RT-qPCR for detecting transfection efficiency of oe-MELK; (c) clone formation assay for cell proliferation; (d) cell viability by CCK8; (e) transwell assay for cell invasion and migration; (f) EdU staining for cell proliferation; (g) Western blot for the level of EMT-related proteins. <span class="nowrap"><svg height="10.7395pt" id="M6" style="vertical-align:-0.6370001pt" version="1.1" viewbox="-0.0498162 -10.1025 58.6784 10.7395" width="58.6784pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,2.179,-5.741)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 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Journal of Immunology Research

fig2

Figure 2

Figure 2: miR-21-5p Inhibits Ferroptosis in Hepatocellular Carcinoma Cells by Regulating the AKT/mTOR Signaling Pathway through MELK 