miR-21-5p Inhibits Ferroptosis in Hepatocellular Carcinoma Cells by Regulating the AKT/mTOR Signaling Pathway through MELK

Figure 4

MELK inhibits liver cancer cell ferroptosis through AKT/mTOR: (a) coimmunoprecipitation to verify the interaction between MELK and AKT; (b) CCK8 for cell viability; (c) Western blot for the level of MELK, AKT, mTOR, p-AKT, and p-mTOR; (d) Western blot for the level of ferroptosis-related proteins GPX4, HO-1, FTH1, and xCT; (e) kit assay for the level of GSH; (f) flow cytometry to detect ROS content; (g) kit assay to detect the Fe²⁺ content, , , .

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