miR-21-5p Inhibits Ferroptosis in Hepatocellular Carcinoma Cells by Regulating the AKT/mTOR Signaling Pathway through MELK

Figure 5

After the addition of an ferroptosis inducer, the effect of miR-21-5p on the ferroptosis of hepatoma cells and the level of EMT was weakened: (a) Western blot analysis of the level of GPX4, HO-1, FTH1, xCT; (b) kit assay for detecting the level of GSH; (c) flow cytometry for ROS content; (d) kit assay to detect Fe²⁺content; (e) clone formation assay for detecting cell proliferation; (f) transwell assay for detecting invasion and migration; (g) EdU staining to detect cell proliferation; (h) Western blot assay of the expression of E-cadherin, vimentin, fibronection, and N-cadherin, , , .

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