Immunoinformatics Approach to Design a Chimeric CD70-Peptide Vaccine against Renal Cell Carcinoma

Table 4

Final selected HTL epitopes from CD70 protein for constructing multiepitope vaccine with their corresponding MHC Class II alleles and immunogenic traits.

Protein name	Position	Epitope sequence	No. of allels	Allels	NetMHCIIpan average rank scores	Allergenicity	Antigenicity	Toxicity	IFN-γ –induction
CD70 (Homo sapiens)	54–72	GWDVAELQLNHTGPQQDPR	6	DRB4_0101; DRB1_1302; DRB3_0202; DRB1_0401; DRB1_0802; HLA-DQA10301-DQB10302	2.71	Nonallergen	1.0262	Non	Inducer
	69–84	QDPRLYWQGGPALGRS	6	DRB1_0101; DRB1_0901; DRB1_1501; HLA-DQA10501-DQB10201; HLA-DQA10501-DQB10301; DRB1_1601	2.45	Nonallergen	0.5512	Non	Inducer

Lower rates show better binding affinity. Higher rate shows high degree of peptide antigenicity.