KIF26B and CREB3L1 Derived from Immunoscore Could Inhibit the Progression of Ovarian Cancer

Figure 6

Knockdown of KIF26B and CREB3L1 gene expression inhibited the migration and invasion ability of SKOV3 cells. (a) The detection of the wound area after 0, 24, and 48 hr of wound and transfection by wound healing. (b) The statistics of wound healing rate after 0, 24, and 48 hr of wound and transfection. The scale bar was 200 μm, the number of repeated experiments was six, and the statistical method was one-way ANOVA followed by Dunnett test for multiple comparisons. (###, Ctrl vs. 10% FBS + siNC group. ,10% FBS + siNC vs. 10% FBS + siK556 or 10% FBS + siC593 group.) (c) The detection of the crystal violet staining of SKOV3 cells that enter the lower chamber by the transwell invasion assays when the expression of KIF26B and CREB3L1 was decreased. (d) The statistical quantification of the results of transwell invasion assay. The scale bar was 100 μm, and the n in the figure represented the number of repeated experiments. The statistical method was one-way ANOVA followed by Dunnett test for multiple comparisons. (ns: nonsignificant, ).

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