PM2.5 Causes Increased Bacterial Invasion by Affecting HBD1 Expression in the Lung

Figure 1

Expression of HBD1 at different conditions within the cell model. (a) ELISA was used to detect the levels of secretory HBD1 in the supernatants of HBE cell cultures in the control, PM2.5, PAO1, and PM2.5 + PAO1 groups (n = 3). (b) RT–qPCR was performed to detect the mRNA levels of HBE in the control, PM2.5, PAO1, and PM2.5 + PAO1 groups of HBE cells (n = 3). (c) Cell lysate smear plate colony count (n = 3). (d) RT–qPCR was performed to detect the mRNA levels of A549 in the control, PM2.5, PAO1, and PM2.5 + PAO1 groups of A549 cells (n = 3). (a, b, d) , , vs. control, ^&&& PAO1 vs. PAO1 + PM2.5 (one-way ANOVA). (c) vs. PAO1, ^&&& PAO1 + PM2.5 vs. HBD1 + PAO1 + PM2.5 (one-way ANOVA).

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