PM2.5 Causes Increased Bacterial Invasion by Affecting HBD1 Expression in the Lung

Figure 4

Mouse model and HBD1 expression. (a) IHC microscopic observation of mouse lung tissue HBD1, (a1–a4) in the order of control, PM2.5 group, PAO1 group, and PAO1 + PM2.5 group (scale bars = 100 µm, n = 6). (b) IHC scoring of lung tissue in mice (n = 6). (c) ELISA for secreted HBD1 in the supernatant of alveolar lavage fluid (n = 6). (d) Detection of DEFB1 mRNA expression in lung tissue homogenates by RT–qPCR (n = 6). (e) Flowchart of the animal model. (f) Survival curves for each group of mice. (g) Colony count of mouse lung grinding homogenate dilution coated plates (n = 6). (b–d) , , vs. control, ^&&P <0.01, ^&&& PAO1 vs. PAO1 + PM2.5 (one-way ANOVA). (g) PAO1 vs. PAO1 + PM2.5, ^&&& PM2.5 + PAO1 vs. HBD1 + PM2.5 + PAO1 (one-way ANOVA).

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