Lipid Bilayer Composition Affects Transmembrane Protein Orientation and Function
Figure 4
Mean phosphate (PO4) detected (nmoL ± SD) in liposomes (lipid-only liposomes), Na+ K+-ATPase alone (enzyme-only), and proteoliposomes (liposomes containing ATPase) made by filtration with a lipid : protein ratio of 20 : 1 () or 10 : 1 () after 60 minutes of incubation. Liposomes and proteoliposomes contained 0.5 μg lipid/μl prior to vesicle production, while proteoliposomes and enzyme-only contained 0.025 μg Na+ K+-ATPase/μl for 20 : 1 and 0.05 μg Na+ K+-ATPase/μl for 10 : 1. PO4 in each well was detected by reading colour intensity at 750 nm. Significant differences within lipid : protein are marked by .
