The Interaction of Plasma Sialylated and Desialylated Lipoproteins with Collagen from the Intima and Media of Uninvolved and Atherosclerotic Human Aorta

Table 1

Binding of sialylated and desialylated VLDL to collagen isolated from two layers of the intima and the media of uninvolved and atherosclerotic areas of human aorta.

Lipoprotein	Collagen-bound apoB, pg/well
	PG-rich layer	Musculoelastic layer	Media
Uninvolved area
Sialylated VLDL	414 ± 37	143 ± 10	150 ± 4*
Desialylated VLDL	650 ± 58*	299 ± 20*	328 ± 4
Initial lesion
Sialylated VLDL	435 ± 10	126 ± 10	120 ± 12*
Desialylated VLDL	598 ± 18*	310 ± 20*	304 ± 15
Fatty streak
Sialylated VLDL	135 ± 11^&	113 ± 10	130 ± 7
Desialylated VLDL	233 ± 10^*&	339 ± 10*	332 ± 8*
Fibroatheroma
Sialylated VLDL	180 ± 12^&	127 ± 3	165 ± 4
Desialylated VLDL	383 ± 20^*&	336 ± 30*	440 ± 9*

Each well of 96-well microtiter plates was coated with collagen and washed as described in Section 2. The binding of VLDL (10 μg/mL) was performed at 37°C for 2 h. Values are the means ± S.E.M (n = 4); *significant difference from sialylated VLDL (𝑃<0.05); ^&significant difference from uninvolved region (𝑃<0.05).