The Interaction of Plasma Sialylated and Desialylated Lipoproteins with Collagen from the Intima and Media of Uninvolved and Atherosclerotic Human Aorta

Table 4

Binding of sialylated and desialylated HDL to collagen isolated from two layers of the intima and the media of uninvolved and atherosclerotic areas of human aorta.

Lipoprotein	Collagen-bound apoA1, pg/well
	PG-rich layer	Musculoelastic layer	Media
Uninvolved area
Sialylated HDL	279 ± 4	312 ± 12	274 ± 50
Desialylated HDL	320 ± 3*	319 ± 3	276 ± 13
Initial lesion
Sialylated HDL	256 ± 15	299 ± 14	369 ± 16
Desialylated HDL	524 ± 34^&*	280 ± 9	378 ± 13
Fatty streak
Sialylated HDL	256 ± 27	313 ± 17	320 ± 43
Desialylated HDL	431 ± 26^&*	290 ± 17	310 ± 16
Fibroatheroma
Sialylated HDL	382 ± 36^&	500 ± 18^&	497 ± 15^&
Desialylated HDL	290 ± 12	508 ± 14^&	242 ± 17*

Each well of 96-well microtiter plates was coated with collagen and washed as described in Section 2. The binding of HDL (10 μg/mL) was performed at 37°C for 2 h. Values are the means ± S.E.M (n = 4); *significant difference from sialylated HDL (𝑃<0.05); ^&significant difference from uninvolved region (𝑃<0.05).