Enzymatically Modified Low-Density Lipoprotein Is Recognized by C1q and Activates the Classical Complement Pathway

Figure 3

Cosedimentation analysis of the interaction between the C1q globular domain and artificial lipid vesicles. Vesicles were prepared from PC alone, PC : cholesterol (1 : 2, w/w), PC : linoleic acid (1 : 2, w/w), or PC : cholesterol : linoleic acid (1 : 2 : 2, w/w/w). Each type of vesicle was incubated with the C1q globular domain (C1q GR), and binding was measured from the relative amount of C1q GR associated with the vesicles in the ultracentrifugation pellet, as determined by SDS-PAGE analysis of the pellet (P) and supernatant (S) fractions. Chol: cholesterol; LA: linoleic acid; PC: phosphatidylcholine (taken from [15], with permission).