LysoPC and PAF Trigger Arachidonic Acid Release by Divergent Signaling Mechanisms in Monocytes

<table>PAF and [<sup>3</sup>H]AA release in human monocytes. Prelabeled THP-1 cells were stimulated with PAF, and [<sup>3</sup>H]AA release was measured by liquid scintillation counting. (a) The time course of PAF- (35 <i>μ</i>M) mediated [<sup>3</sup>H]AA. (b) PAF stimulation for 5 min mediates [<sup>3</sup>H]AA release in a dose-dependent matter. (c) Shows inhibition of PAF-induced [<sup>3</sup>H]AA by the sPLA<sub>2</sub> inhibitor SB203347 (10 <i>μ</i>M) and the cPLA<sub>2</sub> inhibitor MAFP (10 <i>μ</i>M). The PAF concentration used is 35 <i>μ</i>M. Data are expressed as means ± SD of triplicate determinations within separate experiments. Asterisks indicate that values are statistically different from PAF-treated cells (*).</table>

Journal of Lipids

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Figure 1

Figure 1: LysoPC and PAF Trigger Arachidonic Acid Release by Divergent Signaling Mechanisms in Monocytes 