LysoPC and PAF Trigger Arachidonic Acid Release by Divergent Signaling Mechanisms in Monocytes
Figure 2
Expression of PLD isoforms in THP-1 cells. RT-PCR with primers specific for different PLD isoforms (PLD1a 638 bp, PLD1b 533 bp, and PLD2 710 bp) was performed on total RNA isolated from undifferentiated THP-1, THP-1 cells differentiated for 72 h and 120 h (lanes 2,3, and 4, resp.; lane 1 is the positive control). Band intensities were calculated using BioRad image analysis software and fold induction of PLD mRNAs (normalized to b-actin) relative to undifferentiated cells is shown in the lower panel.
