LysoPC and PAF Trigger Arachidonic Acid Release by Divergent Signaling Mechanisms in Monocytes

Figure 4

LysoPC and PAF stimulate PLD activity. (a) [¹⁴C]choline prelabeled THP-1 cells were stimulated with lysoPC or PAF for two minutes, and [¹⁴C]choline released in the medium was measured after TLC separation. (b) and (c) [¹⁴C]choline prelabeled THP-1 cells were preincubated with WEB2170, SB203347, or PTX before (b) lysoPC or (c) PAF challenge. Data are expressed as means ± SD of triplicate determinations within separate experiments. Asterisks indicate that values are statistically different from PAF- or lysoPC-treated cells (*).

(a)

(b)

(c)