Regulation of Serum Response Factor and Adiponectin by PPAR Agonist Docosahexaenoic Acid
Figure 1
Effects of DHA on SRF and adiponectin in 3T3-F442A adipocytes. 3T3-F442A adipocytes differentiated as described in Methods were treated with 25 M DHA, 25 M DHA plus 1.5 M GW9662 or 1.5 M GW9662 in DMEM containing 1% FBS for 16 h. Cells were lysed and separated on 10% SDS-PAGE followed by Western blotting with specific antibody. (a) Blots were analyzed with SRF C-terminus antibody. (d) Bar graph shows quantification using densitometric analysis of 65 kDa SRF protein. Data in the bar graph represents means of three independent experiments ( , ). (b) The same blot was analyzed with -actin antibody to examine equal loading of proteins. (c) The same samples were also examined for the expression of adiponectin. Blots were probed with adiponectin antibody; bar graph shows quantitation of adiponectin protein using densitometric analysis and is expressed as percent of control. (e) Data in the bar graph represents means of three independent experiments (, ).
