Regulation of Serum Response Factor and Adiponectin by PPAR Agonist Docosahexaenoic Acid
Figure 2
SRF expression in mouse adipocytes treated with DHA. Equal volumes (1-2 ml) of freshly isolated adipocytes were incubated in DMEM-HEPES supplemented with penicillin-streptomycin, containing 1% BSA. Adipocytes were treated as specified control, 25 M DHA, 25 M DHA plus 1.5 M GW9662, or 1.5 M GW9662 alone for 16 h. Cells and medium were separated and analyzed for SRF expression. (a) Western blots of SRF expression in mouse adipocytes. (d) Data in the adjacent bar graph represents one of two experiments done in duplicate(, ). (b) The blot from part A was probed with -actin antibody. (c) Western blot of SRF expression in adipocyte conditioned medium. Densitometric analysis of SRF expression in cells, and medium is expressed as a percent of control. (e) The bar graph represents means of all replicate experiments (, ).
