Regulation of Serum Response Factor and Adiponectin by PPAR Agonist Docosahexaenoic Acid
Figure 3
Fractionation of adipocyte medium following treatment with DHA or DHA in the presence of PPAR inhibitor GW 9662. Mouse adipocytes were treated: control (C), 25M DHA (D), or 25 M DHA plus 1.5 M GW9662 (DI). Equal volumes of medium secreted from treatments were fractionated on sucrose gradients and separated into 11 equal fractions as described in Methods. (a) Equal volumes of each fraction were separated on SDS-PAGE gels and analyzed by Western blotting using adiponectin, Flotillin, or SRF antibody as described in Methods. (b) Bar graph represents densitometric analysis of change in adiponectin and SRF expression following DHA treatment in each fraction: DHA/Control.
