Research Article

Novel Polyoxyethylene-Containing Glycolipids Are Synthesized in Corynebacterium matruchotii and Mycobacterium smegmatis Cultured in the Presence of Tween 80

Figure 1

Partial MALDI mass spectrum of TDCM-like glycolipid from C. matruchotii grown in the presence of Tween 80. The glycolipids in the chloroform/methanol extract were purified by silica gel and Sephadex LH20 column chromatography. Molecular ion peaks (MNa+) at m/z 613 to 921 represent series-3 glycolipids and those at m/z 997 to 1595 represent series-2 glycolipids. TDCM (m/z 1425) represents a glycolipid containing two -corynomycolates.
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