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Journal of Lipids
Volume 2011, Article ID 676535, 12 pages
Research Article

Novel Polyoxyethylene-Containing Glycolipids Are Synthesized in Corynebacterium matruchotii and Mycobacterium smegmatis Cultured in the Presence of Tween 80

1Mycobacteriology Research Laboratory, William S. Middleton Memorial Veterans Hospital, Madison, WI 53705, USA
2Chemical Biology & Therapeutics, St. Jude Children's Research Hospital, Memphis, TN 38105, USA
3Department of Chemistry, University of Wisconsin, Madison, WI 53706, USA
4Department of Bacteriology, University of Wisconsin, Madison, WI 53706, USA

Received 16 March 2010; Accepted 21 April 2010

Academic Editor: Sampath Parthasarathy

Copyright © 2011 Cindy Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Highly purified series-2 glycolipid prepared from the chloroform/methanol extract of C. matruchotii cultured in the presence of Tween 80 was analyzed by MALDI mass spectrometry. From the resulting spectrum (Figure S1), MNa+ peaks at m/z 1111 and 1331 were lifted for tendem mass spectrometry (MS-MS).

Figure S1: MALDI mass spectrum of series-2 glycolipid from C. matruchotii grown in the presence of Tween 80. This spectrum shows the structrual series of the major series-2B peaks at m/z 1067-1859 and minor series-2A peaks at m/z 1259-1567.

  1. Supplementary Figure